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Nucleic Acids Res. 2017 Jun 20;45(11):e99. doi: 10.1093/nar/gkx177.

Predicting the impact of non-coding variants on DNA methylation.

Author information

1
Computer Science and Artificial Intelligence Lab, Massachusetts Institute of Technology Cambridge, MA 02142, USA.

Abstract

DNA methylation plays a crucial role in the establishment of tissue-specific gene expression and the regulation of key biological processes. However, our present inability to predict the effect of genome sequence variation on DNA methylation precludes a comprehensive assessment of the consequences of non-coding variation. We introduce CpGenie, a sequence-based framework that learns a regulatory code of DNA methylation using a deep convolutional neural network and uses this network to predict the impact of sequence variation on proximal CpG site DNA methylation. CpGenie produces allele-specific DNA methylation prediction with single-nucleotide sensitivity that enables accurate prediction of methylation quantitative trait loci (meQTL). We demonstrate that CpGenie prioritizes validated GWAS SNPs, and contributes to the prediction of functional non-coding variants, including expression quantitative trait loci (eQTL) and disease-associated mutations. CpGenie is publicly available to assist in identifying and interpreting regulatory non-coding variants.

PMID:
28334830
PMCID:
PMC5499808
DOI:
10.1093/nar/gkx177
[Indexed for MEDLINE]
Free PMC Article

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