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Gene. 1987;61(3):299-306.

An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a.

Author information

1
Advanced Genetic Sciences Inc., Oakland, CA 94608.

Abstract

We describe the construction and utilization of a new mobilizable cosmid vector. Using this vector, mobilizable libraries of bacterial DNA can be efficiently made without a need for size fractionation of target DNA. The low stability of this vector in Pseudomonas fluorescens makes it useful in a rapid strategy, which is not dependent on plasmid incompatibility, for recombining transposon-induced mutations into the bacterial chromosome.

PMID:
2833429
DOI:
10.1016/0378-1119(87)90193-4
[Indexed for MEDLINE]

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