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Virology. 1988 Apr;163(2):391-6.

A new SV40 mutant that encodes a small fragment of T antigen transforms established rat and mouse cells.

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Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.


We have constructed a new SV40 mutant, T147, that makes a large T antigen that is only 147 amino acids long. We show that the T147 T antigen is a phosphoprotein that is as stable as wild-type T antigen and that the SV40 viral origin binding activity of the T147 T antigen is reduced at least 100-fold relative to that of wild-type T antigen. Most importantly, we demonstrate that cloned T147 DNA transforms rat F111 cells to anchorage independence as efficiently as cloned wild-type SV40 DNA and that cloned T147 DNA also efficiently transforms C3H10T1/2 mouse cells in a focus assay.

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