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Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jun 15;181:47-54. doi: 10.1016/j.saa.2017.03.032. Epub 2017 Mar 16.

Antibacterial properties of amino acid functionalized silver nanoparticles decorated on graphene oxide sheets.

Author information

1
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492010, (C.G.), India.
2
Department of Chemistry, Govt. Madhav Science P. G. College, Ujjain 456010, (M.P.), India.
3
School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492010, (C.G.), India.
4
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492010, (C.G.), India. Electronic address: manmohanchem@gmail.com.

Abstract

Graphene oxide (GO) sheets decorated with amino acid L-cysteine (L-cys) functionalized silver nanoparticles (GO-L-cys-Ag) was synthesized by AgNO3, trisodium citrate, and NaBH4. GO-L-cys-Ag nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, ultraviolet-visible (UV-vis) absorption spectra, which demonstrated that a diameter of L-cys-AgNPs compactly deposited on GO. Antibacterial activity tests of GO-L-cys-Ag nanocomposite were carried out using Escherichia coli MTCC 1687 and Staphylococcus aureus MTCC 3160 as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage on antibacterial activity of GO-L-cys-Ag nanocomposite was examined by plate count, well diffusion and broth dilution methods. Morphological observation of bacterial cells by scanning electron microscope (SEM) showed that GO-L-cys-Ag nanocomposite was more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. The above technique establish that the bactericidal property of GO-L-cys-Ag nanocomposite with wide range of applications in biomedical science.

KEYWORDS:

Antibacterial activity; GO-L-cys-Ag nanocomposite; Graphene oxide; L-cys-AgNPs; SEM

PMID:
28329722
DOI:
10.1016/j.saa.2017.03.032
[Indexed for MEDLINE]

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