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Gigascience. 2017 May 1;6(5):1-7. doi: 10.1093/gigascience/gix018.

Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q.

Author information

1
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China.
2
BGI-Shenzhen, Shenzhen 518083, China.
3
School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA.
4
Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
5
Department of Entomology, Volcani Center, Bet Dagan 5025001, Israel.
6
Key Lab of Bio-pesticide Creation and Application, South China Agricultural University, Guangzhou 510642, China.
7
College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China.
8
Cirad, UMR PVBMT, Saint-Pierre, La Réunion, France.
9
Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UK.
10
Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
11
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
12
Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
13
School of Horticulture and Plant Protection and Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China.
14
Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China.
15
Collaborative Innovation Center of Henan Grain Crops, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
16
Department of Biological Sciences, University of Rhode Island, Kingston, Rhode Island 02881, USA.
17
College of Life Sciences, Hubei University, Wuhan 430062, China.
18
United States Department of Agriculture, Agricultural Research Service, Corn Insects & Crop Genetics Research Unit, Ames, IA 50011, USA.
19
Department of Entomology, S-225 Agricultural Science Center North, University of Kentucky, Lexington, KY 40546-0091, USA.

Abstract

The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future 'pan-genomic' comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management.

KEYWORDS:

Annotation; Assembly; Genomics; Whitefly Bemisia tabaci

PMID:
28327996
PMCID:
PMC5467035
DOI:
10.1093/gigascience/gix018
[Indexed for MEDLINE]
Free PMC Article

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