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Biosci Biotechnol Biochem. 2017 Jul;81(7):1274-1278. doi: 10.1080/09168451.2017.1303361. Epub 2017 Mar 20.

Pseudomonas aeruginosa cytochrome c551 denaturation by five systematic urea derivatives that differ in the alkyl chain length.

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a Graduate School of Biosphere Science , Hiroshima University , Higashi-Hiroshima , Japan.
b Graduate School of Science Technology and Innovation , Kobe University , Kobe , Japan.
c Graduate School of Engineering Science , Osaka University , Osaka , Japan.
d Elements Strategy Initiative for Catalysts and Batteries , Kyoto University , Kyoto , Japan.


Reversible denaturation of Pseudomonas aeruginosa cytochrome c551 (PAc551) could be followed using five systematic urea derivatives that differ in the alkyl chain length, i.e. urea, N-methylurea (MU), N-ethylurea (EU), N-propylurea (PU), and N-butylurea (BU). The BU concentration was the lowest required for the PAc551 denaturation, those of PU, EU, MU, and urea being gradually higher. Furthermore, the accessible surface area difference upon PAc551 denaturation caused by BU was found to be the highest, those by PU, EU, MU, and urea being gradually lower. These findings indicate that urea derivatives with longer alkyl chains are stronger denaturants. In this study, as many as five systematic urea derivatives could be applied for the reversible denaturation of a single protein, PAc551, for the first time, and the effects of the alkyl chain length on protein denaturation were systematically verified by means of thermodynamic parameters.


Pseudomonas aeruginosa; cytochrome c; protein denaturation; urea derivatives

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