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J AOAC Int. 2017 May 1;100(3):640-646. doi: 10.5740/jaoacint.17-0047. Epub 2017 Mar 18.

Determination of Triazines and Triazoles in Grapes Using Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization High-Resolution Mass Spectrometry.

Author information

1
Council of Scientific and Industrial Research-National Chemical Laboratory, Chemical Engineering Division, Dr. Homi Bhabha Rd, Pune 411 008, India.
2
Indian Council of Agricultural Research, National Research Centre for Grapes, National Referral Laboratory, PO Manjri Farm, Solapur Rd, Pune 412 307, Maharashtra, India.

Abstract

A chromatography-free atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry (AP-MALDI HRMS) method is described for the simultaneous and quantitative detection of triazines and triazoles in grapes. The analytes were detected reproducibly with high mass accuracy (mass error within 5 ppm) and further confirmed by collision-induced dissociation fragmentation in tandem MS. The LODs and LOQs for all the analytes were found to be in the nanogram per gram level (15-20 ng/g LOQ). Internal standard-normalized high-resolution accurate mass-extracted (HR-AM) peak intensities of the detected ions were used to generate the concentration response curves. Linearity (with R2 values around 0.99) was obtained for these curves within a concentration range of 20-200 ng/g of the individual analytes. The accuracy and precision of the method were further established using QC samples. Validation and performance comparison of the AP-MALDI HRMS method with an existing standard method using LC with triple quadrupole MS was carried out (evaluating sensitivity, accuracy, precision, and analysis time) using 20 table-grape field samples after QuEChERS extraction.

PMID:
28315638
DOI:
10.5740/jaoacint.17-0047
[Indexed for MEDLINE]

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