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Cytometry A. 2017 May;91(5):424-432. doi: 10.1002/cyto.a.23083. Epub 2017 Mar 17.

Characterization of dormant and active human cancer cells by quantitative phase imaging.

Guo P1,2, Huang J1,2, Moses MA1,2.

Author information

1
Vascular Biology Program, Boston Children's Hospital, 300 Longwood Avenue, Boston, Massachusetts, 02115.
2
Department of Surgery, Harvard Medical School and Boston Children's Hospital, 300 Longwood Avenue, Boston, Massachusetts, 02115.

Abstract

The switch of tumor cells from a dormant, non-angiogenic phenotype to an active, angiogenic phenotype is a critical step in early cancer progression. To date, relatively little is known about the cellular behaviors of angiogenic and non-angiogenic tumor cell phenotypes. In this study, holographic imaging cytometry, a quantitative phase imaging (QPI) technique was used to continuously and non-invasively analyze, quantify, and compare a panel of fundamental cellular behaviors of angiogenic and non-angiogenic human osteosarcoma cells (KHOS) in a simple and economical way. Results revealed that angiogenic KHOS cells (KHOS-A) have significantly higher cell motility speeds than their non-angiogenic counterpart (KHOS-N) while no difference in their cell proliferation rates and cell cycle lengths were observed. KHOS-A cells were also found to have significantly smaller cell areas and greater cell optical thicknesses when compared with the non-angiogenic KHOS-N cells. No difference in average cell volumes was observed. These studies demonstrate that the morphology and behavior of angiogenic and non-angiogenic cells can be continuously, efficiently, and non-invasively monitored using a simple, quantitative, and economical system that does not require tedious and time-consuming assays to provide useful information about tumor dormancy.

KEYWORDS:

cell cycle; cell migration; cell morphology; cell phenotype; holographic microscopy; osteosarcoma; quantitative phase imaging; tumor angiogenesis; tumor dormancy

PMID:
28314083
DOI:
10.1002/cyto.a.23083
[Indexed for MEDLINE]
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