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Cell. 1988 Feb 12;52(3):397-404.

Cis-acting negative control of DNA replication in eukaryotic cells.

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Division of Basic Sciences, Fred Hutchinson Cancer Center, Seattle, Washington 98104.


We show that in stable monkey cell lines, the replication of a chimeric SV40-BPV episomal replicon occurs once and only once per cell cycle. The copy number of this episome is stably maintained even when an excess of the limiting initiation factor T antigen is provided. These experiments therefore uncover a cis-acting negative control mechanism whereby replication control is not focused on limiting the activity of positive factors; rather, replication is permitted in unreplicated replicons but is actively prevented, in cis, in replicons that have already been duplicated. We also find that the initiation factor SV40 T antigen remains associated with its SV-BPV episomal template in a kinetically stable and potentially heritable state.

[Indexed for MEDLINE]

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