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Vet Microbiol. 1987 Oct;15(1-2):11-8.

Genetic reassortment of bluetongue virus serotype 11 strains in the bovine.

Author information

1
Department of Veterinary Pathology, University of California, Davis 95616.

Abstract

Reassortants of bluetongue virus Serotype 11 (BTV-11) were isolated from a yearling heifer experimentally infected with two electrophoretically different strains (UC-2 and UC-8) by subcutaneous inoculation. Viruses were recovered by direct titration of sonicated blood samples onto Vero cell monolayers, which were overlaid with agarose and later plaque purified. The parental electropherotype of UC-8 was identified as the predominant virus strain during the infection; UC-2 was not isolated. UC-2 infectivity was shown by reassortants which contained genome segments that were identical in migration pattern to the parental UC-2 electropherotype. The observations demonstrate that segmental reassortment can occur during mixed infections in the bovine, between strains of the same BTV serotype.

PMID:
2830703
[Indexed for MEDLINE]

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