Format

Send to

Choose Destination
J Asthma. 2017 Dec;54(10):1012-1018. doi: 10.1080/02770903.2017.1292282. Epub 2017 Feb 21.

Asthma causes inflammation of human pulmonary arteries and decreases vasodilatation induced by prostaglandin I2 analogs.

Author information

1
a Laboratory of Cardiovascular Genetic and Nutritional Diseases , University Setif 1 , Algeria.
2
b Faculty of Medicine, University Setif 1 , Algeria.
3
c Laboratory of Functional Genomics in Atherothrombosis , Lyon , France.

Erratum in

Abstract

OBJECTIVE:

Asthma is a chronic inflammatory disease associated with increased cardiovascular events. This study assesses the presence of inflammation and the vascular reactivity of pulmonary arteries in patients with acute asthma.

METHODS:

Rings of human pulmonary arteries obtained from non-asthmatic and asthmatic patients were set up in organ bath for vascular tone monitoring. Reactivity was induced by vasoconstrictor and vasodilator agents. Protein expression of inflammatory markers was detected by western blot. Prostanoid releases and cyclic adenosine monophosphate (cAMP) levels were quantified using specific enzymatic kits.

RESULTS:

Protein expression of cluster of differentiation 68, intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and cyclooxygenase-2 was significantly increased in arteries obtained from asthmatic patients. These effects were accompanied by an alteration of vasodilatation induced by iloprost and treprostinil, a decrease in cAMP levels and an increase in prostaglandin (PG) E2 and PGI2 synthesis. The use of forskolin (50 µmol/L) has restored the vasodilatation and cAMP release. No difference was observed between the two groups in reactivity induced by norepinephrine, angiotensin II, PGE2, KCl, sodium nitroprusside, and acetylcholine.

CONCLUSION:

Acute asthma causes inflammation of pulmonary arteries and decreases vasodilation induced by PGI2 analogs through the impairment of cAMP pathway.

KEYWORDS:

Asthma; prostaglandins; pulmonary arteries; vasoconstriction; vasodilatation

PMID:
28306357
DOI:
10.1080/02770903.2017.1292282
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center