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ACS Chem Biol. 2017 Mar 17;12(3):611-621. doi: 10.1021/acschembio.6b01089. Epub 2017 Feb 13.

Tools for Studying Glycans: Recent Advances in Chemoenzymatic Glycan Labeling.

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Department of Chemical Physiology, The Scripps Research Institute , La Jolla, California 92037, United States.
Vassar College , 124 Raymond Ave, Poughkeepsie, New York 12604, United States.


The study of cellular glycosylation presents many challenges due, in large part, to the nontemplated nature of glycan biosynthesis and glycans' structural complexity. Chemoenzymatic glycan labeling (CeGL) has emerged as a new technique to address the limitations of existing methods for glycan detection. CeGL combines glycosyltransferases and unnatural nucleotide sugar donors equipped with a bioorthogonal chemical tag to directly label specific glycan acceptor substrates in situ within biological samples. This article reviews the current CeGL strategies that are available to characterize cell-surface and intracellular glycans. Applications include imaging glycan expression status in live cells and tissue samples, proteomic analysis of glycoproteins, and target validation. Combined with genetic and biochemical tools, CeGL provides new opportunities to elucidate the functional roles of glycans in human health and disease.

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