Format

Send to

Choose Destination
Cell. 1988 Jan 29;52(2):161-7.

GAL4 activates gene expression in mammalian cells.

Author information

1
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

Abstract

GAL4, a protein that activates transcription in yeast, is shown to activate the mouse mammary tumor virus promoter in mammalian cells. Activation depends upon a GAL4-binding sequence inserted upstream of the gene. Deletion mutants of GAL4 bearing one or both of the "activating regions" required for activation in yeast also activate transcription in mammalian cells. A derivative of GAL4 that binds to DNA but cannot activate transcription in yeast also fails to activate transcription in mammalian cells. We also show that GAL4 and the glucocorticoid receptor activate the mouse mammary tumor virus promoter synergistically.

PMID:
2830021
DOI:
10.1016/0092-8674(88)90504-1
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center