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J Clin Microbiol. 2017 May;55(5):1249-1254. doi: 10.1128/JCM.00358-17. Epub 2017 Mar 15.

Real-Time Sequencing of Mycobacterium tuberculosis: Are We There Yet?

Lee RS1,2, Pai M3,4,5.

Author information

1
Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.
2
Microbiological Diagnostic Unit Public Health Laboratory, The University of Melbourne, Melbourne, Victoria, Australia.
3
McGill International TB Centre, Montréal, Québec, Canada madhukar.pai@mcgill.ca.
4
McGill Global Health Programs, McGill University, Montréal, Québec, Canada.
5
Department of Epidemiology, Biostatistics and Occupational Health, McGill University, Montréal, Québec, Canada.

Abstract

Whole-genome sequencing has taken a leading role in epidemiologic studies of tuberculosis, but thus far, its real-time clinical utility has been low, in part because of the requirement for culture. In their report in this issue, Votintseva et al. (A. A. Votintseva, P. Bradley, L. Pankhurst, C. del Ojo Elias, M. Loose, K. Nilgiriwala, A. Chatterjee, E. G. Smith, N. Sanderson, T. M. Walker, M. R. Morgan, D. H. Wyllie, A. S. Walker, T. E. A. Peto, D. W. Crook, and Z. Iqbal, J Clin Microbiol 55:1285-1298, 2017, https://doi.org/10.1128/JCM.02483-16) present a new method for extracting Mycobacterium tuberculosis DNA directly from smear-positive respiratory samples, making it feasible to generate drug resistance predictions and phylogenetic trees in 44 h with the Illumina MiSeq. They also illustrate the potential for a <24-h turnaround time from DNA extraction to clinically relevant results with Illumina MiniSeq and Oxford Nanopore Technologies MinION. We comment on the promise and limitations of these approaches.

PMID:
28298449
PMCID:
PMC5405243
DOI:
10.1128/JCM.00358-17
[Indexed for MEDLINE]
Free PMC Article

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