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J Cell Mol Med. 2017 Sep;21(9):1803-1814. doi: 10.1111/jcmm.13101. Epub 2017 Mar 14.

Circulating long non-coding RNAs NRON and MHRT as novel predictive biomarkers of heart failure.

Author information

1
Department of Pharmacology, (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, China.
2
Department of Epidemiology and Biostatistics, Public Health School, Harbin Medical University, Harbin, Heilongjiang, China.
3
Department of Cardiology, the First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China.
4
Division of Pathophysiology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China and the Key Laboratory of Cardiovascular Research, Ministry of Education), Harbin Medical University, Harbin, Heilongjiang, China.
5
Department of Cardiology, the Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China.
6
Institute of Clinical Pharmacology, the Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China.
7
Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Melbourne, Australia.

Abstract

This study sought to evaluate the potential of circulating long non-coding RNAs (lncRNAs) as biomarkers for heart failure (HF). We measured the circulating levels of 13 individual lncRNAs which are known to be relevant to cardiovascular disease in the plasma samples from 72 HF patients and 60 non-HF control participants using real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) methods. We found that out of the 13 lncRNAs tested, non-coding repressor of NFAT (NRON) and myosin heavy-chain-associated RNA transcripts (MHRT) had significantly higher plasma levels in HF than in non-HF subjects: 3.17 ± 0.30 versus 1.0 ± 0.07 for NRON (P < 0.0001) and 1.66 ± 0.14 versus 1.0 ± 0.12 for MHRT (P < 0.0001). The area under the ROC curve was 0.865 for NRON and 0.702 for MHRT. Univariate and multivariate analyses identified NRON and MHRT as independent predictors for HF. Spearman's rank correlation analysis showed that NRON was negatively correlated with HDL and positively correlated with LDH, whereas MHRT was positively correlated with AST and LDH. Hence, elevation of circulating NRON and MHRT predicts HF and may be considered as novel biomarkers of HF.

KEYWORDS:

MHRT ; NRON ; LncRNA; heart failure; plasma

PMID:
28296001
PMCID:
PMC5571539
DOI:
10.1111/jcmm.13101
[Indexed for MEDLINE]
Free PMC Article

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