A lysosome targetable fluorescent probe for endogenous imaging of hydrogen peroxide in living cells

Shahi Imam Reja; Muskan Gupta; Neha Gupta; Vandana Bhalla; Puja Ohri; Gurcharan Kaur; Manoj Kumar

doi:10.1039/c6cc09127j

A lysosome targetable fluorescent probe for endogenous imaging of hydrogen peroxide in living cells

Chem Commun (Camb). 2017 Mar 28;53(26):3701-3704. doi: 10.1039/c6cc09127j.

Authors

Shahi Imam Reja¹, Muskan Gupta², Neha Gupta¹, Vandana Bhalla¹, Puja Ohri³, Gurcharan Kaur², Manoj Kumar¹

Affiliations

¹ Department of Chemistry, UGC Sponsored Centre for Advanced Studies-II, Guru Nanak Dev University, Amritsar, Punjab, India. mksharmaa@yahoo.co.in.
² Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, India.
³ Department of Zoology, Guru Nanak Dev University, India.

PMID: 28294228
DOI: 10.1039/c6cc09127j

Abstract

A lysosome targetable naphthalimide based fluorescent probe (LyNC) has been designed and synthesized which detects hydrogen peroxide (H₂O₂) with high selectivity and sensitivity in brain tissues and in living nematodes among various ROS/RNS tested. Further, the probe LyNC was successfully employed in exogenous and endogenous imaging of H₂O₂ in living cell lines.

MeSH terms

Animals
Cell Line, Tumor
Cell Survival
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Glioma / chemistry*
Hydrogen Peroxide / analysis*
Lysosomes / chemistry*
Molecular Structure
Naphthalimides / chemical synthesis
Naphthalimides / chemistry*
Optical Imaging / methods*
Rats

Substances

Fluorescent Dyes
Naphthalimides
Hydrogen Peroxide