Format

Send to

Choose Destination
Development. 2017 Mar 15;144(6):998-1007. doi: 10.1242/dev.140905.

Dissecting the stem cell niche with organoid models: an engineering-based approach.

Author information

1
Department of Pharmaceutical Chemistry, University of California, San Francisco, 600 16th Street, Box 2280, San Francisco, CA 94158, USA.
2
Graduate Program in Chemistry and Chemical Biology, University of California at San Francisco, San Francisco, CA 94158, USA.
3
Department of Pharmaceutical Chemistry, University of California, San Francisco, 600 16th Street, Box 2280, San Francisco, CA 94158, USA zev.gartner@ucsf.edu.

Abstract

For many tissues, single resident stem cells grown in vitro under appropriate three-dimensional conditions can produce outgrowths known as organoids. These tissues recapitulate much of the cell composition and architecture of the in vivo organ from which they derive, including the formation of a stem cell niche. This has facilitated the systematic experimental manipulation and single-cell, high-throughput imaging of stem cells within their respective niches. Furthermore, emerging technologies now make it possible to engineer organoids from purified cellular and extracellular components to directly model and test stem cell-niche interactions. In this Review, we discuss how organoids have been used to identify and characterize stem cell-niche interactions and uncover new niche components, focusing on three adult-derived organoid systems. We also describe new approaches to reconstitute organoids from purified cellular components, and discuss how this technology can help to address fundamental questions about the adult stem cell niche.

KEYWORDS:

Cell culture; Engineer; Model; Niche; Organoid; Stem

PMID:
28292846
PMCID:
PMC5358107
DOI:
10.1242/dev.140905
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center