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Nat Commun. 2017 Mar 14;8:14750. doi: 10.1038/ncomms14750.

An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.

Author information

1
School of Biochemistry, University of Bristol, Bristol BS8 1TD, UK.
2
Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
3
Bristol Institute for Transfusion Sciences, National Health Service Blood and Transplant (NHSBT), Bristol BS34 7QH, UK.
4
NIHR Blood and Transplant Research Unit, University of Bristol, Bristol BS8 1TD, UK.
5
School of Cellular and Molecular Medicine, University of Bristol, Bristol BS8 1TD, UK.
6
Department of Research and Development, Central Blood Institute, Japanese Red Cross Society, Tokyo 135-8521, Japan.
7
Cell Engineering Division, RIKEN BioResource Center, Ibaraki 305-0074, Japan.

Abstract

With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors do not yet provide a sustainable supply, and current systems using pluripotent stem cells as progenitors do not generate viable red cells. We have taken an alternative approach, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply of red cells. The immortalized cells differentiate efficiently into mature, functional reticulocytes that can be isolated by filtration. Extensive characterization has not revealed any differences between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the molecular level, and importantly no aberrant protein expression. We demonstrate a feasible approach to the manufacture of red cells for clinical use from in vitro culture.

PMID:
28290447
PMCID:
PMC5355882
DOI:
10.1038/ncomms14750
[Indexed for MEDLINE]
Free PMC Article

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