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Eur J Appl Physiol. 2017 May;117(5):931-941. doi: 10.1007/s00421-017-3582-4. Epub 2017 Mar 13.

Intestinal fatty acid-binding protein and gut permeability responses to exercise.

Author information

1
Department of Infection, Immunity and Inflammation, College of Medicine, Biological Sciences and Psychology, University of Leicester, Leicester, UK.
2
Centre for Immunobiology, Blizard Institute, Barts and The London School of Medicine, Queen Mary University of London, London, UK.
3
Peninsula Medical School, Plymouth University, The John Bull Building, Tamar Science Park, Research Way, Plymouth, UK.
4
Lincoln Institute for Health, University of Lincoln, Lincoln, UK.
5
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.
6
Endurance Research Group, School of Sport and Exercise Sciences, University of Kent, Medway Campus, Chatham Maritime, ME4 4AG, UK. g.davison@kent.ac.uk.

Abstract

PURPOSE:

Intestinal cell damage due to physiological stressors (e.g. heat, oxidative, hypoperfusion/ischaemic) may contribute to increased intestinal permeability. The aim of this study was to assess changes in plasma intestinal fatty acid-binding protein (I-FABP) in response to exercise (with bovine colostrum supplementation, Col, positive control) and compare this to intestinal barrier integrity/permeability (5 h urinary lactulose/rhamnose ratio, L/R).

METHODS:

In a double-blind, placebo-controlled, crossover design, 18 males completed two experimental arms (14 days of 20 g/day supplementation with Col or placebo, Plac). For each arm participants performed two baseline (resting) intestinal permeability assessments (L/R) pre-supplementation and one post-exercise following supplementation. Blood samples were collected pre- and post-exercise to determine I-FABP concentration.

RESULTS:

Two-way repeated measures ANOVA revealed an arm × time interaction for L/R and I-FABP (P < 0.001). Post hoc analyses showed urinary L/R increased post-exercise in Plac (273% of pre, P < 0.001) and Col (148% of pre, P < 0.001) with post-exercise values significantly lower with Col (P < 0.001). Plasma I-FABP increased post-exercise in Plac (191% of pre-exercise, P = 0.002) but not in the Col arm (107%, P = 0.862) with post-exercise values significantly lower with Col (P = 0.013). Correlations between the increase in I-FABP and L/R were evident for visit one (P = 0.044) but not visit two (P = 0.200) although overall plots/patterns do appear similar for each.

CONCLUSION:

These findings suggest that exercise-induced intestinal cellular damage/injury is partly implicated in changes in permeability but other factors must also contribute.

KEYWORDS:

Bovine colostrum; Cell damage; Cellular injury; Core temperature; Intestinal permeability; Strenuous exercise; Urinary L/R

PMID:
28290057
PMCID:
PMC5388720
DOI:
10.1007/s00421-017-3582-4
[Indexed for MEDLINE]
Free PMC Article

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