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Cancer Immunol Immunother. 2017 Jun;66(6):777-786. doi: 10.1007/s00262-017-1982-2. Epub 2017 Mar 13.

Expression patterns of programmed death-ligand 1 in esophageal adenocarcinomas: comparison between primary tumors and metastases.

Author information

1
Institute of Pathology, University of Bern, Murtenstr. 31, 3010, Bern, Switzerland.
2
Department of Visceral Surgery and Medicine, Inselspital University Hospital Bern, University Bern, 3010, Bern, Switzerland.
3
Institute of Pathology, Technical University of Munich, 81675, Munich, Germany.
4
Research Unit Analytical Pathology, Helmholtz Center Munich, German Research Center for Environmental Health, 85764, Oberschleißheim, Germany.
5
Institute of Pathology, University of Bern, Murtenstr. 31, 3010, Bern, Switzerland. rupert.langer@pathology.unibe.ch.

Abstract

Expression analysis of programmed death-ligand 1 (PD-L1) may be helpful in guiding clinical decisions for immune checkpoint inhibition therapy, but testing by immunohistochemistry may be hampered by heterogeneous staining patterns within tumors and expression changes during metastatic course. PD-L1 expression (clone SP142) was investigated in esophageal adenocarcinomas using tissue microarrays (TMA) from 112 primary resected tumors, preoperative biopsies and full slide sections from a subset of these cases (n = 24), corresponding lymph node (n = 55) and distant metastases (n = 17). PD-L1 expression was scored as 0.1-1, >1, >5, >50% positive membranous staining of tumor cells and any positive staining of tumor-associated inflammatory infiltrates and/or stroma cells. There was a significant correlation with overall PD-L1 expression between the full slide sections and the TMA (p = 0.001), but not with the corresponding biopsies. PD-L1 expression in tumor cells >1% was detected in 8.0% of cases (9/112) and 51.8% of cases (58/112) in tumor-associated inflammatory infiltrates and/or stroma cells of primary tumors. Epithelial expression in metastases was found in 5.6% of cases (4/72) and immune cell expression in 18.1% of cases (13/72), but did not correlate with the expression pattern in the primary tumor. Overall PD-L1 expression in the primary tumor did not influence survival. However, PD-L1 expression was correlated with the number of CD3+ tumor-infiltrating lymphocytes in the tumor center, and a combinational score of PD-L1 status/CD3+ tumor-infiltrating lymphocytes was correlated with patients' overall survival.

KEYWORDS:

Esophageal adenocarcinoma; Immunohistochemistry; Metastases; PD-L1

PMID:
28289861
DOI:
10.1007/s00262-017-1982-2
[Indexed for MEDLINE]

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