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Development. 2017 Apr 15;144(8):1462-1471. doi: 10.1242/dev.144907. Epub 2017 Mar 13.

Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration.

Author information

1
Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, Tatzberg 47 01307, Germany jan.kaslin@monash.edu.
2
Australian Regenerative Medicine Institute, Monash University, Innovation Walk 15, Lvl1, 3800, Clayton, Melbourne, Australia.
3
Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, Tatzberg 47 01307, Germany.

Abstract

Zebrafish can regenerate after brain injury, and the regenerative process is driven by resident stem cells. Stem cells are heterogeneous in the vertebrate brain, but the significance of having heterogeneous stem cells in regeneration is not understood. Limited availability of specific stem cells might impair the regeneration of particular cell lineages. We studied regeneration of the adult zebrafish cerebellum, which contains two major stem and progenitor cell types: ventricular zone and neuroepithelial cells. Using conditional lineage tracing we demonstrate that cerebellar regeneration depends on the availability of specific stem cells. Radial glia-like cells are thought to be the predominant stem cell type in homeostasis and after injury. However, we find that radial glia-like cells play a minor role in adult cerebellar neurogenesis and in recovery after injury. Instead, we find that neuroepithelial cells are the predominant stem cell type supporting cerebellar regeneration after injury. Zebrafish are able to regenerate many, but not all, cell types in the cerebellum, which emphasizes the need to understand the contribution of different adult neural stem and progenitor cell subtypes in the vertebrate central nervous system.

KEYWORDS:

Cerebellum; Neural stem cell; Neurogenesis; Radial glia; Regeneration; Zebrafish

PMID:
28289134
DOI:
10.1242/dev.144907
[Indexed for MEDLINE]
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