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Nat Methods. 2017 Apr;14(4):391-394. doi: 10.1038/nmeth.4222. Epub 2017 Mar 13.

Optogenetic control with a photocleavable protein, PhoCl.

Author information

1
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
2
Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada.
3
Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
4
Department of Biosystems Science and Engineering, ETH Z├╝rich, Basel, Switzerland.
5
Alberta Glycomics Centre, University of Alberta, Edmonton, Alberta, Canada.

Abstract

To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.

PMID:
28288123
DOI:
10.1038/nmeth.4222
[Indexed for MEDLINE]

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