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J Mol Biol. 1987 Oct 20;197(4):729-36.

Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase.

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Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.


The gene gyrA of Escherichia coli, which encodes the A subunit of DNA gyrase (topoisomerase II), has been cloned and a region of approximately 3300 base-pairs sequenced. An open reading frame of 2625 nucleotides coding for a protein of 97,000 Mr is located. The peptide weight of the subunit predicted from this open reading frame is in close agreement with previously published estimates of that of the A subunit. There is a "TATAAT" promoter motif located 44 bases upstream from the first "ATG" of the open reading frame. The amino acid sequence derived from the nucleotide sequence is about 50% homologous with that derived from the Bacillus subtilis gyrA gene sequence, with several regions showing greater than 90% homology.

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