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Antiviral Res. 2017 Jun;142:16-20. doi: 10.1016/j.antiviral.2017.03.003. Epub 2017 Mar 7.

5-Hydroxytyrosol inhibits HIV-1 replication in primary cells of the lower and upper female reproductive tract.

Author information

1
AIDS Immunopathogenesis Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy. Electronic address: elisa.saba@gmail.com.
2
Reproductive Sciences Laboratory, Division of Genetics and Cell Biology, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy. Electronic address: panina.paola@hsr.it.
3
Viral Pathogens and Biosafety Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy. Electronic address: pagani.isabel@hsr.it.
4
Department of Obstetrics and Gynecology, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy; Vita-Salute San Raffaele University, School of Medicine, Via Olgettina 58, 20132, Milano, Italy. Electronic address: origoni.massimo@hsr.it.
5
Department of Obstetrics and Gynecology, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy; Vita-Salute San Raffaele University, School of Medicine, Via Olgettina 58, 20132, Milano, Italy. Electronic address: candiani.massimo@hsr.it.
6
Vita-Salute San Raffaele University, School of Medicine, Via Olgettina 58, 20132, Milano, Italy; Pathology Unit, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy. Electronic address: doglioni.claudio@hsr.it.
7
Pathology Unit, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy. Electronic address: taccagni.gianluca@hsr.it.
8
Viral Pathogens and Biosafety Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy. Electronic address: ghezzi.silvia@hsr.it.
9
AIDS Immunopathology Department, National Centre of Microbiology, Instituto de Salud Carlos III. Cra Majadahonda a Pozuelo, 28220, Majadahonda, Madrid, Spain. Electronic address: ppalcami@isciii.es.
10
Viral Pathogens and Biosafety Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy. Electronic address: vicenzi.elisa@hsr.it.
11
AIDS Immunopathogenesis Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy; Vita-Salute San Raffaele University, School of Medicine, Via Olgettina 58, 20132, Milano, Italy. Electronic address: poli.guido@hsr.it.

Abstract

We investigated the potential anti-HIV-1 activity of the candidate microbicide 5-hydroxytyrosol (5-HT) both in primary human cervical tissue explants (CTE), established from tissues of women undergoing histerectomy, and in endometrium-associated leukocytes (EAL). CTE were exposed to either the laboratory-adapted HIV-1BaL or to primary viral isolates in the presence or absence of 5-HT or 3TC/lamivudine as control and were then monitored for 12 days in terms of HIV-1 p24 Gag antigen production in culture supernatants. HIV-1BaL replication was also evaluated in EAL by reverse transcriptase (RT) activity. The highest nontoxic concentrations of 5-HT (200 and 100 μM for CTE and EAL, respectively) exerted a significant inhibitory effect on virus replication in both primary cell systems. 5-HT did not cause significant alterations of the activation profile of CD4+ and CD8+ T cells, in terms of CD4, CCR5, CD25, CD69 and HLA-DR expression, although it decreased the percentage of CD38+CD8+ T cells. Thus, 5-HT deserves consideration as a potential candidate microbicide for preventing HIV-1 transmission or curtailing its replication in the female reproductive tract.

KEYWORDS:

5-Hydroxytyrosol; CD38; Endometrium-associated leukocytes; HIV-1; Human cervical tissue explants; Microbicide

PMID:
28286236
DOI:
10.1016/j.antiviral.2017.03.003
[Indexed for MEDLINE]

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