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Structure. 2017 Apr 4;25(4):650-654.e2. doi: 10.1016/j.str.2017.02.003. Epub 2017 Mar 9.

Recognition of Histone H3K14 Acylation by MORF.

Author information

1
Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
2
Epigenetics Program, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
3
Department of Epigenetics and Molecular Carcinogenesis, Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
4
Laval University Cancer Research Center, CHU de Québec-UL Research Center-Oncology Axis, Quebec City, QC G1R 3S3, Canada.
5
Epigenetics Program, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: bgarci@mail.med.upenn.edu.
6
Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA. Electronic address: tatiana.kutateladze@ucdenver.edu.

Abstract

The monocytic leukemia zinc-finger protein-related factor (MORF) is a transcriptional coactivator and a catalytic subunit of the lysine acetyltransferase complex implicated in cancer and developmental diseases. We have previously shown that the double plant homeodomain finger (DPF) of MORF is capable of binding to acetylated histone H3. Here we demonstrate that the DPF of MORF recognizes many newly identified acylation marks. The mass spectrometry study provides comprehensive analysis of H3K14 acylation states in vitro and in vivo. The crystal structure of the MORF DPF-H3K14butyryl complex offers insight into the selectivity of this reader toward lipophilic acyllysine substrates. Together, our findings support the mechanism by which the acetyltransferase MORF promotes spreading of histone acylation.

KEYWORDS:

DPF; MORF; PTM; double PHD finger; epigenetic; histone binding; lysine acylation

PMID:
28286003
PMCID:
PMC5415407
DOI:
10.1016/j.str.2017.02.003
[Indexed for MEDLINE]
Free PMC Article

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