Format

Send to

Choose Destination
Nat Chem. 2017 Feb;9(2):157-163. doi: 10.1038/nchem.2616. Epub 2016 Oct 10.

A synthetic redox biofilm made from metalloprotein-prion domain chimera nanowires.

Altamura L1,2,3, Horvath C1,2,3, Rengaraj S1,2,3,4,5, Rongier A1,2,3,6,7,8, Elouarzaki K4,5, Gondran C4,5, Maçon AL1,2,3, Vendrely C9,10, Bouchiat V11, Fontecave M1,2,3,12, Mariolle D13, Rannou P6,7,8, Le Goff A4,5, Duraffourg N1,2,3, Holzinger M4,5, Forge V1,2,3.

Author information

1
Université Grenoble Alpes, BIG-LCBM, F-38000 Grenoble, France.
2
CNRS, BIG-LCBM, F-38000 Grenoble, France.
3
CEA, BIG-LCBM, F-38000 Grenoble, France.
4
Université Grenoble Alpes, DCM UMR 5250, F-38000 Grenoble, France.
5
CNRS, DCM UMR 5250, F-38000 Grenoble, France.
6
Université Grenoble Alpes, INAC-SPrAM, F-38000 Grenoble, France.
7
CNRS, INAC-SPrAM, F-38000 Grenoble, France.
8
CEA, INAC-SPrAM, F-38000 Grenoble, France.
9
ERRMECe, I-MAT FD4122, Université de Cergy-Pontoise, 2 avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France.
10
LMGP, CNRS UMR 5628, 3 parvis Louis Néel, 38016 Grenoble, France.
11
Institut Néel, CNRS-UJF-INP, 38042 Grenoble Cedex 09, France.
12
Laboratoire de Chimie des Processus Biologiques, UMR 8229 CNRS, Université Pierre et Marie Curie - Paris 6, Collège de France. 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France.
13
CEA, LETI, MINATEC Campus, F-38054 Grenoble, France.

Abstract

Engineering bioelectronic components and set-ups that mimic natural systems is extremely challenging. Here we report the design of a protein-only redox film inspired by the architecture of bacterial electroactive biofilms. The nanowire scaffold is formed using a chimeric protein that results from the attachment of a prion domain to a rubredoxin (Rd) that acts as an electron carrier. The prion domain self-assembles into stable fibres and provides a suitable arrangement of redox metal centres in Rd to permit electron transport. This results in highly organized films, able to transport electrons over several micrometres through a network of bionanowires. We demonstrate that our bionanowires can be used as electron-transfer mediators to build a bioelectrode for the electrocatalytic oxygen reduction by laccase. This approach opens opportunities for the engineering of protein-only electron mediators (with tunable redox potentials and optimized interactions with enzymes) and applications in the field of protein-only bioelectrodes.

PMID:
28282052
DOI:
10.1038/nchem.2616
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center