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J Environ Sci Health B. 2017 Jun 3;52(6):410-417. doi: 10.1080/03601234.2017.1293449. Epub 2017 Mar 10.

In vitro cytotoxicity assessment of roundup (glyphosate) in L-02 hepatocytes.

Author information

1
a Changsha Center for Disease Control and Prevention , Changsha , PR China.
2
b Hunan Provincial Center for Disease Control and Prevention , Changsha , PR China.
3
c Department of Health Toxicology , Xiangya School of Public Health, Central South University , Changsha , PR China.

Abstract

The goal of the present study was to elucidate the in vitro cytotoxicity of Roundup and to reveal the possible related mechanisms in L-02 hepatocytes. By detecting reactive oxygen species (ROS) production, glutathione (GSH)/superoxide dismutase (SOD) levels, mitochondrial permeability transition pore (PTP) open rate, apoptosis-inducing factor (AIF) release, intracellular Ca2+ concentration, and alanine aminotransferease (ALT)/aspartate aminotransferase (AST) leakage, we determined that Roundup induced anti-oxidant system inhibition, mitochondria damage, DNA damage, membrane integrity and permeability changes, and apoptosis in L-02 hepatocytes. By revealing the mechanistic insights of Roundup-induced cytotoxicity, our results are valuable for the design of preventive and therapeutic strategies for the occupational population exposed to Roundup and other pesticides.

KEYWORDS:

Cytotoxicity; L-02 hepatocytes; ROS; Roundup; glyphosate

PMID:
28281894
DOI:
10.1080/03601234.2017.1293449
[Indexed for MEDLINE]

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