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Science. 2017 Mar 10;355(6329):1081-1084. doi: 10.1126/science.aah5403. Epub 2017 Mar 9.

Mutation of a nucleosome compaction region disrupts Polycomb-mediated axial patterning.

Author information

1
Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
2
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
3
Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
4
Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA. kingston@molbio.mgh.harvard.edu.

Abstract

Nucleosomes play important structural and regulatory roles by tightly wrapping the DNA that constitutes the metazoan genome. The Polycomb group (PcG) proteins modulate nucleosomes to maintain repression of key developmental genes, including Hox genes whose temporal and spatial expression is tightly regulated to guide patterning of the anterior-posterior body axis. CBX2, a component of the mammalian Polycomb repressive complex 1 (PRC1), contains a compaction region that has the biochemically defined activity of bridging adjacent nucleosomes. Here, we demonstrate that a functional compaction region is necessary for proper body patterning, because mutating this region leads to homeotic transformations similar to those observed with PcG loss-of-function mutations. We propose that CBX2-driven nucleosome compaction is a key mechanism by which PcG proteins maintain gene silencing during mouse development.

PMID:
28280206
PMCID:
PMC5503153
DOI:
10.1126/science.aah5403
[Indexed for MEDLINE]
Free PMC Article

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