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RNA Biol. 2017 Sep 2;14(9):1209-1222. doi: 10.1080/15476286.2017.1295204. Epub 2017 Feb 23.

Wobble uridine modifications-a reason to live, a reason to die?!

Author information

1
a Institut für Biologie, FG Mikrobiologie , Universität Kassel , Germany.
2
b Max Planck Institute for Molecular Biomedicine , Germany.
3
c Cells-in-Motion Cluster of Excellence , University of Münster , Münster , Germany.
4
d Medical Faculty , University of Münster , Albert-Schweitzer-Campus 1, Münster , Germany.

Abstract

Wobble uridines (U34) are generally modified in all species. U34 modifications can be essential in metazoans but are not required for viability in fungi. In this review, we provide an overview on the types of modifications and how they affect the physico-chemical properties of wobble uridines. We describe the molecular machinery required to introduce these modifications into tRNA posttranscriptionally and discuss how posttranslational regulation may affect the activity of the modifying enzymes. We highlight the activity of anticodon specific RNases that target U34 containing tRNA. Finally, we discuss how defects in wobble uridine modifications lead to phenotypes in different species. Importantly, this review will mainly focus on the cytoplasmic tRNAs of eukaryotes. A recent review has extensively covered their bacterial and mitochondrial counterparts. 1.

KEYWORDS:

Anticodon wobble base; RNases; mRNA translation; protein homeostasis; stress responses; tRNA modification

PMID:
28277930
PMCID:
PMC5699542
DOI:
10.1080/15476286.2017.1295204
[Indexed for MEDLINE]
Free PMC Article

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