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Sci Rep. 2017 Mar 7;7(1):97. doi: 10.1038/s41598-017-00162-3.

Region specific Raman spectroscopy analysis of the femoral head reveals that trabecular bone is unlikely to contribute to non-traumatic osteonecrosis.

Author information

1
Lille University, Littoral Côte d'Opale University, EA 4490, PMOI, Physiopathologie des Maladies Osseuses Inflammatoires, F-59000, Lille, France. pascart.tristan@ghicl.net.
2
Department of Rheumatology, Saint-Philibert Hospital, Lille University, F-59160, Lomme, France. pascart.tristan@ghicl.net.
3
Lille University, Littoral Côte d'Opale University, EA 4490, PMOI, Physiopathologie des Maladies Osseuses Inflammatoires, F-59000, Lille, France.
4
Department of Orthopaedic Surgery, Lille University Hospital, Lille University, F-59000, Lille, France.
5
Department of Anatomopathology, Saint-Philibert Hospital, Lille University, F-59160, Lomme, France.
6
Department of biostatistics, Saint-Philibert Hospital, Lille University, F-59160, Lomme, France.
7
Department of Radiology,Saint-Philibert Hospital, Lille University, F-59160, Lomme, France.
8
Department of Rheumatology, Lille University Hospital, Lille University, F-59000, Lille, France.
9
Department of Radiology, Lille University Hospital, Lille University, F-59000, Lille, France.

Abstract

Non-traumatic osteonecrosis (ON) of the femoral head is a common disease affecting a young population as the peak age of diagnosis is in the 40 s. The natural history of non-traumatic ON leads to a collapse of the femoral head requiring prosthetic replacement in a 60% of cases. Although trabecular bone involvement in the collapse is suspected, the underlying modifications induced at a molecular level have not been explored in humans. Here, we examine changes in the molecular composition and structure of bone as evaluated by Raman spectroscopy in human end-stage ON. Comparing samples from femoral heads harvested from 11 patients and 11 cadaveric controls, we show that the mineral and organic chemical composition of trabecular bone in ON is not modified apart from age-related differences. We also show that the molecular composition in the necrotic part of the femoral head is not different from the composition of the remaining 'healthy' trabecular bone of the femoral head. These findings support that quality of trabecular bone is not modified during ON despite extensive bone marrow necrosis and osteocyte death observed even in the 'healthy' zones on histological examination.

PMID:
28273910
PMCID:
PMC5427816
DOI:
10.1038/s41598-017-00162-3
[Indexed for MEDLINE]
Free PMC Article

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