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Curr Opin Biotechnol. 2017 Dec;48:8-14. doi: 10.1016/j.copbio.2017.02.006. Epub 2017 Mar 6.

Optogenetic methods in drug screening: technologies and applications.

Author information

1
AXXAM SpA, via Meucci 3, 20091 Bresso, Milan, Italy.
2
Institute of Science and Technology (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria. Electronic address: harald@ist.ac.at.

Abstract

The optogenetic revolution enabled spatially-precise and temporally-precise control over protein function, signaling pathway activation, and animal behavior with tremendous success in the dissection of signaling networks and neural circuits. Very recently, optogenetic methods have been paired with optical reporters in novel drug screening platforms. In these all-optical platforms, light remotely activated ion channels and kinases thereby obviating the use of electrophysiology or reagents. Consequences were remarkable operational simplicity, throughput, and cost-effectiveness that culminated in the identification of new drug candidates. These blueprints for all-optical assays also revealed potential pitfalls and inspire all-optical variants of other screens, such as those that aim at better understanding dynamic drug action or orphan protein function.

PMID:
28273648
DOI:
10.1016/j.copbio.2017.02.006
[Indexed for MEDLINE]

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