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Am J Sports Med. 2017 Mar;45(3):666-675. doi: 10.1177/0363546516668835. Epub 2016 Oct 23.

Comparison of 2 Different Formulations of Artificial Bone for a Hybrid Implant With a Tissue-Engineered Construct Derived From Synovial Mesenchymal Stem Cells: A Study Using a Rabbit Osteochondral Defect Model.

Author information

1
Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, Osaka, Japan.
2
Biomechanics Laboratory, Department of Mechanical Engineering, Kogakuin University, Tokyo, Japan.
3
Division of Human Mechatronics Systems, Faculty of System Design, Tokyo Metropolitan University, Tokyo, Japan.
4
Department of Orthopaedic Surgery, Kansai Rosai Hospital, Amagasaki, Japan.
5
Graduate School of Comprehensive Rehabilitation, Osaka Prefecture University, Osaka, Japan.
6
McCaig Institute for Bone & Joint Health, University of Calgary, Calgary, Alberta, Canada.
7
Orthopaedic Arthroscopic Surgery International, Milan, Italy.
8
Institute for Medical Science in Sports, Osaka Health Science University, Osaka, Japan.
9
Center for Advanced Medical Engineering and Informatics, Osaka University, Osaka, Japan.

Abstract

BACKGROUND:

Previously, we developed a hybrid implant composed of hydroxyapatite (HA)-based artificial bone coupled with a mesenchymal stem cell (MSC)-based scaffold-free tissue-engineered construct (TEC) and demonstrated its feasibility for osteochondral repair. Beta-tricalcium phosphate (βTCP) may be a promising alternative to HA, as it is a highly biocompatible material and is resorbed more rapidly than HA in vivo.

HYPOTHESIS:

A βTCP-based hybrid TEC implant will exhibit superior osteochondral repair when directly compared with an HA-based hybrid implant, as tested using a rabbit osteochondral defect model.

STUDY DESIGN:

Controlled laboratory study.

METHODS:

Osteochondral defects were created in the femoral groove of skeletally mature rabbits. The TEC and artificial bone, using either HA or βTCP with the same porosities and similar mechanical properties, were hybridized and then implanted in the defects. A histological evaluation and microindentation testing were performed for the assessment of repair tissue.

RESULTS:

Osteochondral defects treated with the TEC/βTCP implants showed more rapid subchondral bone repair at 1 month, but the cartilaginous tissue deteriorated over time out to 6 months after implantation. Osteochondral defects treated with the TEC/HA implants maintained good histological quality out to 6 months after implantation and also exhibited better biomechanical properties at 6 months as compared with the TEC/βTCP implants.

CONCLUSION:

Contrary to our hypothesis, the TEC/HA hybrid implant facilitated better osteochondral repair than did the TEC/βTCP implant. The results of the present study suggest the importance of a stable restoration of subchondral bone for long-term effective osteochondral repair rather than rapid remodeling of subchondral bone.

CLINICAL RELEVANCE:

This study contributes to the future selection of suitable materials for patients with osteochondral lesions.

KEYWORDS:

artificial bone; beta-tricalcium phosphate; hydroxyapatite; mesenchymal stem cell; osteochondral repair; scaffold free

PMID:
28272938
DOI:
10.1177/0363546516668835
[Indexed for MEDLINE]

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