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Genes Dev. 2017 Feb 1;31(3):241-246. doi: 10.1101/gad.292169.116.

Cbx3 maintains lineage specificity during neural differentiation.

Author information

1
Department of Biological Chemistry, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.
2
Department of Neurobiology, Shantou University Medical College, Shantou 515041, China.
3
Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles, Los Angeles, California 90095, USA.
4
Department of Neurobiology, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles California 90095, USA.

Abstract

Chromobox homolog 3 (Cbx3/heterochromatin protein 1γ [HP1γ]) stimulates cell differentiation, but its mechanism is unknown. We found that Cbx3 binds to gene promoters upon differentiation of murine embryonic stem cells (ESCs) to neural progenitor cells (NPCs) and recruits the Mediator subunit Med26. RNAi knockdown of either Cbx3 or Med26 inhibits neural differentiation while up-regulating genes involved in mesodermal lineage decisions. Thus, Cbx3 and Med26 together ensure the fidelity of lineage specification by enhancing the expression of neural genes and down-regulating genes specific to alternative fates.

KEYWORDS:

Cbx3; Med26; embryonic stem cell; mesoderm; neural precursor; preinitiation complex

PMID:
28270516
PMCID:
PMC5358721
DOI:
10.1101/gad.292169.116
[Indexed for MEDLINE]
Free PMC Article

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