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Conf Proc IEEE Eng Med Biol Soc. 2016 Aug;2016:3922-3925. doi: 10.1109/EMBC.2016.7591585.

Multimodal embryonic imaging using optical coherence tomography, selective plane illumination microscopy, and optical projection tomography.

Abstract

The murine model is commonly utilized for studying developmental diseases. Different optical techniques have been developed to image mouse embryos, but each has its own set of limitations and restrictions. In this study, we compare the performance of the well-established technique of optical coherence tomography (OCT) to the relatively new methods of selective plane illumination microscopy (SPIM) and optical projection tomography (OPT) to assess murine embryonic development. OCT can provide label free high resolution images of the mouse embryo, but suffers from light attenuation that limits visualization of deeper structures. SPIM is able to image shallow regions with great detail utilizing fluorescent contrast. OPT can provide superior imaging depth, and can also use fluorescence labels but, it requires samples to be fixed and cleared before imaging. OCT requires no modification of the embryo, and thus, can be used in vivo and in utero. In this study, we compare the efficacy of OCT, SPIM, and OPT for imaging murine embryonic development. The data demonstrate the superior capability of SPIM and OPT for imaging fine structures with high resolution while only OCT can provide structural and functional imaging of live embryos with micrometer scale resolution.

PMID:
28269143
DOI:
10.1109/EMBC.2016.7591585
[Indexed for MEDLINE]

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