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Adv Healthc Mater. 2017 May;6(10). doi: 10.1002/adhm.201601170. Epub 2017 Mar 7.

Generating Multicompartmental 3D Biological Constructs Interfaced through Sequential Injections in Microfluidic Devices.

Author information

1
Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133, Milano, Italy.
2
Cell and Tissue Engineering Lab, IRCCS Istituto Ortopedico Galeazzi, 20161, Milano, Italy.
3
Regenerative Medicine Technologies Lab, Ente Ospedaliero Cantonale, 6900, Lugano, Switzerland.
4
Swiss Institute for Regenerative Medicine, 6900, Lugano, Switzerland.
5
Cardiocentro Ticino, 6900, Lugano, Switzerland.

Abstract

A novel technique is presented for molding and culturing composite 3D cellular constructs within microfluidic channels. The method is based on the use of removable molding polydimethylsiloxane (PDMS) inserts, which allow to selectively and incrementally generate composite 3D constructs featuring different cell types and/or biomaterials, with a high spatial control. The authors generate constructs made of either stacked hydrogels, with uniform horizontal interfaces, or flanked hydrogels with vertical interfaces. The authors also show how this technique can be employed to create custom-shaped endothelial barriers and monolayers directly interfaced with 3D cellular constructs. This method dramatically improves the significance of in vitro 3D biological models, enhancing mimicry and enabling for controlled studies of complex biological districts.

KEYWORDS:

biofabrication; composite 3D constructs; microfluidics; microtissues, organs-on-chip

PMID:
28267277
DOI:
10.1002/adhm.201601170
[Indexed for MEDLINE]

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