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J Biochem Mol Toxicol. 2017 Jul;31(7). doi: 10.1002/jbt.21900. Epub 2017 Mar 7.

Polydatin inhibits cell proliferation and induces apoptosis in laryngeal cancer and HeLa cells via suppression of the PDGF/AKT signaling pathway.

Author information

1
Department of Otolaryngology, Huaihe Hospital of Henan University, Kaifeng, 475000, People's Republic of China.
2
Department of Obstetrics and Gynecology, Huaihe Hospital of Henan University, Kaifeng, 475000, People's Republic of China.
3
Department of Otolaryngology, The First Affiliated Hospital of Henan University, Kaifeng, 475000, People's Republic of China.

Abstract

Polydatin (PD), a stilbene compound extracted from Polygonum cuspidatum, is suggested to possess anti-cancer activities, including inhibition of cell proliferation, cell cycle arrest, and induction of apoptosis. The platelet-derived growth factor (PDGF)/AKT signaling pathway plays complex roles in tumor suppression. However, the effect of PD on the PDGF/AKT signaling pathway in laryngeal cancer and HeLa cells has not been explored. MTT assay and flow cytometry showed that PD inhibited cell proliferation and induced apoptosis in Hep-2 and AMC-HN-8 cells. Western blot analysis indicated that PD inhibited the expression levels of PDGF-B and phosphorylated AKT (p-AKT) in both cells. Treatment of PDGF-B siRNA or PDGFR inhibitor found that after the PDGF signaling was inactivated, p-AKT expression was significantly decreased in Hep-2 cells. Tumor xenograft experiment in nude mice indicated PD significantly inhibited the growth of Hep-2 cells in vivo. In conclusion, PD inhibited cell proliferation and induced apoptosis in laryngeal cancer and HeLa cells via inactivation of the PDGF/AKT signaling pathway.

KEYWORDS:

HeLa cells; PDGF/AKT signaling pathway; apoptosis; cell proliferation; laryngeal cancer; polydatin

PMID:
28266802
DOI:
10.1002/jbt.21900
[Indexed for MEDLINE]

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