Format

Send to

Choose Destination
J Cell Sci. 2018 Jan 29;131(2). pii: jcs200733. doi: 10.1242/jcs.200733.

The plant i-AAA protease controls the turnover of an essential mitochondrial protein import component.

Author information

1
Faculty of Biotechnology, University of Wroclaw, F. Joliot-Curie 14A, Wroclaw 50-383, Poland magdalena.opalinska@uwr.edu.pl hanna.janska@uwr.edu.pl.
2
Faculty of Biotechnology, University of Wroclaw, F. Joliot-Curie 14A, Wroclaw 50-383, Poland.
3
Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley Western Australia 6009, Australia.
4
School of Molecular Sciences, University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.

Abstract

Mitochondria are multifunctional organelles that play a central role in energy metabolism. Owing to the life-essential functions of these organelles, mitochondrial content, quality and dynamics are tightly controlled. Across the species, highly conserved ATP-dependent proteases prevent malfunction of mitochondria through versatile activities. This study focuses on a molecular function of the plant mitochondrial inner membrane-embedded AAA protease (denoted i-AAA) FTSH4, providing its first bona fide substrate. Here, we report that the abundance of the Tim17-2 protein, an essential component of the TIM17:23 translocase (Tim17-2 together with Tim50 and Tim23), is directly controlled by the proteolytic activity of FTSH4. Plants that are lacking functional FTSH4 protease are characterized by significantly enhanced capacity of preprotein import through the TIM17:23-dependent pathway. Taken together, with the observation that FTSH4 prevents accumulation of Tim17-2, our data point towards the role of this i-AAA protease in the regulation of mitochondrial biogenesis in plants.

KEYWORDS:

AAA protease; ATP-dependent proteolysis; Mitochondrial protein import; TIM17:23 translocase

PMID:
28264925
DOI:
10.1242/jcs.200733
Free full text

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center