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Nutrients. 2017 Feb 28;9(3). pii: E212. doi: 10.3390/nu9030212.

Anti-Endometriotic Effects of Pueraria Flower Extract  in Human Endometriotic Cells and Mice.

Kim JH1,2, Woo JH3, Kim HM2, Oh MS1,2, Jang DS1,2, Choi JH4,5.

Author information

1
Department of Life and Nanopharamceutical Sciences, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. hyemi586@gmail.com.
2
College of Pharmacy, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. hyemi586@gmail.com.
3
Department of Life and Nanopharamceutical Sciences, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. radiee@empas.com.
4
Department of Life and Nanopharamceutical Sciences, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. jchoi@khu.ac.kr.
5
College of Pharmacy, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemoon-gu, Seoul 02447, Korea. jchoi@khu.ac.kr.

Abstract

Pueraria flowers have been used as a vegetable and an ingredient for tea and jelly. In this study, we investigated the effects of Pueraria flower extract (PFE) on endometriosis, a common gynaecological disease characterised by local sterile inflammation of peritoneal cavity. PFE suppressed the adhesion of human endometriotic cells 11Z and 12Z to human mesothelial Met5A cells. In addition, PFE significantly inhibited the migration of 11Z and 12Z cells as shown by woundhealing and transwell migration assays. PFE reduced the protein and mRNA levels of matrix metalloproteinase (MMP)-2 and MMP-9 in endometriotic cells. Moreover, extracellular signalregulated kinase (ERK)1/2 was activated by PFE treatment, and an ERK1/2 inhibitor, PD98059, significantly inhibited PFE-inhibited cell migration in endometriotic cells. Furthermore, PFE significantly suppressed endometriotic lesion formation in a mouse model. These data suggest that Pueraria flower is a potential anti-endometriotic agent for the inhibition of endometriotic cell adhesion, migration, and MMP expression.

KEYWORDS:

Pueraria flower;  MMP;  adhesion;  endometriosis;  migration

PMID:
28264481
PMCID:
PMC5372875
DOI:
10.3390/nu9030212
[Indexed for MEDLINE]
Free PMC Article

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