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Nat Biotechnol. 2017 Apr;35(4):347-349. doi: 10.1038/nbt.3804. Epub 2017 Mar 6.

GuideScan software for improved single and paired CRISPR guide RNA design.

Author information

1
Computational Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
2
Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
3
Weill Cornell Graduate School of Medical Sciences of Cornell University, New York, New York, USA.

Abstract

We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.

PMID:
28263296
PMCID:
PMC5607865
DOI:
10.1038/nbt.3804
[Indexed for MEDLINE]
Free PMC Article

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