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Gene. 1987;57(2-3):193-201.

Plasmid and bacteriophage vectors for excision of intact inserts.

Author information

1
LGME-CNRS, Strasbourg, France.

Abstract

Plasmid (pPolyIII) and bacteriophage lambda (EMBL301) vectors are described in which sites for the rare-cutting enzymes SfiI and NotI (8-bp, recognition sequences) flank the polylinker cloning region. Intact DNA inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. General-purpose miniplasmid cloning vectors pPolyI and pPolyII are also described, and the utility of the bacteriophage lambda vector is demonstrated in the construction of a bovine genomic library.

PMID:
2826295
DOI:
10.1016/0378-1119(87)90122-3
[Indexed for MEDLINE]

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