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J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Dec 15;1071:44-48. doi: 10.1016/j.jchromb.2017.01.025. Epub 2017 Feb 24.

From untargeted LC-QTOF analysis to characterisation of opines in abalone adductor muscle: Theory meets practice.

Author information

1
Human Metabolomics, North-West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom 2520, South Africa. Electronic address: Leonie.Venter@nwu.ac.za.
2
Human Metabolomics, North-West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom 2520, South Africa.
3
School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000, South Africa.

Abstract

Abalone have a unique ability to use pyruvate, various amino acids and dehydrogenases, to produce opines as means to prevent the accumulation of NADH during anaerobic conditions. In this study, the theoretical masses, formulae and fragment patterns of butylated opines were used to predict which of these compounds could be found in the abalone adductor muscle using untargeted liquid chromatography quadrupole time-of flight-mass spectrometry. These findings were validated using synthesised opine standards. In essence alanopine, lysopine, strombine and tauropine produced in abalone adductor muscle could be characterised using the highest identification confidence levels.

KEYWORDS:

Abalone; Alanopine; Mass spectrometry; Opines

PMID:
28262445
DOI:
10.1016/j.jchromb.2017.01.025
[Indexed for MEDLINE]

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