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Elife. 2017 Mar 6;6. pii: e22835. doi: 10.7554/eLife.22835.

Novel mechanism of metabolic co-regulation coordinates the biosynthesis of secondary metabolites in Pseudomonas protegens.

Author information

1
Department of Botany and Plant Pathology, Oregon State University, Corvallis, United States.
2
Department of Pharmaceutical Sciences, Oregon State University, Corvallis, United States.
3
US Department of Agriculture, Agricultural Research Service, Horticultural Crops Research Laboratory, Corvallis, United States.

Abstract

Metabolic co-regulation between biosynthetic pathways for secondary metabolites is common in microbes and can play an important role in microbial interactions. Here, we describe a novel mechanism of metabolic co-regulation in which an intermediate in one pathway is converted into signals that activate a second pathway. Our study focused on the co-regulation of 2,4-diacetylphloroglucinol (DAPG) and pyoluteorin, two antimicrobial metabolites produced by the soil bacterium Pseudomonas protegens. We show that an intermediate in DAPG biosynthesis, phloroglucinol, is transformed by a halogenase encoded in the pyoluteorin gene cluster into mono- and di-chlorinated phloroglucinols. The chlorinated phloroglucinols function as intra- and inter-cellular signals that induce the expression of pyoluteorin biosynthetic genes, pyoluteorin production, and pyoluteorin-mediated inhibition of the plant-pathogenic bacterium Erwinia amylovora. This metabolic co-regulation provides a strategy for P. protegens to optimize the deployment of secondary metabolites with distinct roles in cooperative and competitive microbial interactions.

KEYWORDS:

Pseudomonas protegens; biochemistry; infectious disease; metabolic co-regulation; microbiology; secondary metabolite

PMID:
28262092
PMCID:
PMC5395296
DOI:
10.7554/eLife.22835
[Indexed for MEDLINE]
Free PMC Article

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