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Environ Toxicol Pharmacol. 2017 Apr;51:9-15. doi: 10.1016/j.etap.2017.02.016. Epub 2017 Feb 21.

Effects of octylphenol on the expression of StAR, CYP17 and CYP19 in testis of Rana chensinensis.

Author information

1
College of Life Science, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, China; China National Center for Food Safety Risk Assessment, 7 Pan Jia Yuan Nan Li, Beijing 100021, China.
2
College of Life Science, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, China.
3
Second Division of Beijing Armed Police Corps, Beijing 100076, China.
4
College of Life Science, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, China. Electronic address: yu-huizhang@163.com.

Abstract

It has been proposed that a decline in sperm quality is associated with exposure to environmental chemicals with estrogenic activity. Seeking possible explanations for this effect, this study investigated the effects of octylphenol (OP) on the synthesis of steroid hormones in amphibian. Rana chensinensis were exposed to 10-8, 10-7 and 10-6mol/L OP after 10, 20, 30 and 40 days. The cDNA fragments of StAR (274bp), CYP17 (303bp) and CYP19 (322bp) were cloned. In situ hybridization and immunohistochemistry revealed that positive signals of StAR, CYP17, CYP19 mRNA and proteins mainly in the Leydig cells of testes. Real-time PCR showed that up-regulation of StAR and CYP19, and down-regulation of CYP17 after exposure to 10-8, 10-7 and 10-6mol/L OP. The results suggest that OP can alter transcriptions of StAR, CYP17 and CYP19, thus disturb the expressions of StAR, P450c17 and P450arom, thereby adversely affect steroid synthesis.

KEYWORDS:

CYP17; CYP19; Octylphenol; Rana chensinensis; StAR

PMID:
28259069
DOI:
10.1016/j.etap.2017.02.016
[Indexed for MEDLINE]

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