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Angew Chem Int Ed Engl. 2017 Mar 27;56(14):4052-4055. doi: 10.1002/anie.201611729. Epub 2017 Mar 3.

Universal Super-Resolution Multiplexing by DNA Exchange.

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Faculty of Physics and Center for Nanoscience, LMU Munich, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.
Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
Department of Biology II and Center for Nanoscience, LMU Munich, Grosshaderner Strasse 2, 82152, Martinsried, Germany.
Wyss Institute for Biologically Inspired Engineering and Department of Systems Biology, Harvard University, 3 Blackfan Circle, Boston, MA, 02115, USA.


Super-resolution microscopy allows optical imaging below the classical diffraction limit of light with currently up to 20× higher spatial resolution. However, the detection of multiple targets (multiplexing) is still hard to implement and time-consuming to conduct. Here, we report a straightforward sequential multiplexing approach based on the fast exchange of DNA probes which enables efficient and rapid multiplexed target detection with common super-resolution techniques such as (d)STORM, STED, and SIM. We assay our approach using DNA origami nanostructures to quantitatively assess labeling, imaging, and washing efficiency. We furthermore demonstrate the applicability of our approach by imaging multiple protein targets in fixed cells.


DNA nanotechnology; SIM; STED; dSTORM; multiplexing

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