Format

Send to

Choose Destination
Arch Virol. 1987;97(1-2):123-30.

Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody. Brief report.

Author information

1
Institut Armand-Frappier, Centre de recherche en virologie, Qu├ębec, Canada.

Abstract

Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immunofluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3.

PMID:
2825619
DOI:
10.1007/bf01310740
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center