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Methods Mol Biol. 2017;1584:333-346. doi: 10.1007/978-1-4939-6881-7_19.

Probing Synaptic Biomechanics Using Micropillar Arrays.

Author information

1
Department of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.
2
Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, NY, 11973, USA.
3
Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, 1275 York Ave, NY, 10065, USA. husem@mskcc.org.

Abstract

Recent insights into the importance of mechanosensing and force transmission at the immune synapse have spurred increased interest in the mechanical properties of leukocyte cell-cell interactions. In this chapter, we describe an imaging-based strategy for measuring cellular forces that utilizes optically transparent arrays of flexible micropillars. This approach has several distinct advantages over standard traction force microscopy, and we anticipate that it will prove very useful for investigators who wish not only to quantify ligand-induced forces with high spatiotemporal resolution but also to place those forces within the context of a broader cell biological response.

KEYWORDS:

Cytoskeleton; Mechanobiology; Micropillar; Polydimethylsiloxane; Signal transduction; Silicon etching; T cell; Traction force microscopy

PMID:
28255710
DOI:
10.1007/978-1-4939-6881-7_19
[Indexed for MEDLINE]

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