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Clin Transl Oncol. 2017 Aug;19(8):1010-1017. doi: 10.1007/s12094-017-1633-2. Epub 2017 Mar 1.

BRG1 regulation by miR-155 in human leukemia and lymphoma cell lines.

Author information

1
Department of Biochemistry and Molecular Biology III and Immunology, University of Granada, Granada, Spain.
2
GENYO, Centre for Genomics and Oncological Research, Granada, Spain.
3
Department of Biochemistry and Molecular Biology I, University of Granada, Granada, Spain.
4
GENYO, Centre for Genomics and Oncological Research, Granada, Spain. pedromedina@ugr.es.
5
Department of Biochemistry and Molecular Biology I, University of Granada, Granada, Spain. pedromedina@ugr.es.

Abstract

INTRODUCTION/PURPOSE:

BRG1 is a key regulator of leukemia stem cells. Indeed, it has been observed that this type of cells is unable to divide, survive and develop new tumors when BRG1 is down-regulated.

MATERIALS AND METHODS:

We assessed BRG1 and miR-155 expression in 23 leukemia cell lines, and two no pathological lymphocyte samples using qPCR. MiR-155 transfection and western blot were used to analyze the relationship between miR-155 and its validated target, BRG1, by measuring protein expression levels. The effect of miR-155 on cell proliferation and prednisolone sensitivity were studied with resazurin assay.

RESULTS:

BRG1 expression levels could correlate negatively with miR-155 expression levels, at least in Burkitt's lymphoma and diffuse large B cell lymphoma (DLBCL) cell lines. To clarify the role of miR-155 in the regulation of BRG1 expression, we administrated miR-155 mimics in different leukemia/lymphoma cell lines. Our results suggest that miR-155 regulate negatively and significantly the BRG1 expression at least in the MOLT4 cell line.

CONCLUSION:

Our study revealed a previously unknown miR-155 heterogeneity that could result in differences in the treatment with miRNAs in our attempt to inhibit BRG1. However, the expression levels of BRG1 and miR-155, before prednisolone treatment were not statistically significantly associated prednisolone sensitive leukemia cells.

KEYWORDS:

BRG1; Leukemia; Prednisolone; miR-155

PMID:
28251496
DOI:
10.1007/s12094-017-1633-2
[Indexed for MEDLINE]

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