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Biomed Pharmacother. 2017 May;89:515-523. doi: 10.1016/j.biopha.2017.02.050. Epub 2017 Feb 27.

Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer.

Author information

1
School of Pharmacy, The University of Queensland, Brisbane, Queensland, Australia; School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia; Australian Prostate Cancer Research Centre-Qld, Translational Research Institute, Queensland University of Technology, Brisbane, Queensland, Australia.
2
School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia; Australian Prostate Cancer Research Centre-Qld, Translational Research Institute, Queensland University of Technology, Brisbane, Queensland, Australia.
3
School of Pharmacy, The University of Queensland, Brisbane, Queensland, Australia.
4
School of Pharmacy, The University of Queensland, Brisbane, Queensland, Australia. Electronic address: a.hewavitharana@pharmacy.uq.edu.au.

Abstract

BACKGROUND:

Prostate cancer (PCa) is the leading cause of cancer related deaths in men. Carica papaya is a popular tropical plant that has been traditionally used for its nutritional and medicinal properties.

METHODS:

We investigated the anti-proliferative responses of papaya leaf juice (LJP) and its various extracts ("biological"- in vitro digested, "physical"- size exclusion, and "chemical"-solvent extraction) on a range of cell lines representing benign hyperplasia, tumorigenic and normal cells of prostate origin.

RESULTS:

Time course analysis (by 24h, 48h and 72h) of LJP (1-0.1mg/mL) before and after in vitro digestion, and of molecular weight based fractions of LJP showed anti-proliferative responses. The medium polarity fraction of LJP (0.03-0.003mg/mL) after 72h exposure showed potent growth inhibitory (IC50=0.02-0.07mg/mL) and cytotoxic activities on all prostate cells, with the exception of the normal (RWPE-1 and WPMY-1) cells. Flow cytometry analysis showed S phase cell cycle arrest and apoptosis as a possible mechanism for these activities. Medium polar fraction of LJP also inhibited migration and adhesion of metastatic PC-3 cells.

CONCLUSION:

This is the first report suggesting selective anti-proliferative and anti-metastatic attributes of LJP extract against prostatic diseases, including PCa.

KEYWORDS:

Anti-proliferative activity; Extraction; Papaya; Prostate cancer

PMID:
28249253
DOI:
10.1016/j.biopha.2017.02.050
[Indexed for MEDLINE]

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