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Methods Mol Biol. 2017;1556:179-189. doi: 10.1007/978-1-4939-6771-1_9.

Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture.

Low M1,2, Eisner C1,2, Rossi F3,4.

Author information

1
Biomedical Research Centre, University of British Columbia, Vancouver, 2222 Health Sciences Mall, BC, Canada, V6T 1Z3.
2
Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada.
3
Biomedical Research Centre, University of British Columbia, Vancouver, 2222 Health Sciences Mall, BC, Canada, V6T 1Z3. fabio@brc.ubc.ca.
4
Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. fabio@brc.ubc.ca.

Abstract

Fibro/adipogenic progenitors (FAPs ) are tissue-resident mesenchymal stromal cells (MSCs). Current literature supports a role for these cells in the homeostasis and repair of multiple tissues suggesting that FAPs may have extensive therapeutic potential in the treatment of numerous diseases. In this context, it is crucial to establish efficient and reproducible procedures to purify FAP populations from various tissues. Here, we describe a protocol for the isolation and cell culture of FAPs from murine skeletal muscle using fluorescence -activated cell sorting (FACS), which is particularly useful for experiments where high cell purity is an essential requirement. Identification, isolation, and cell culture of FAPs represent powerful tools that will help us to understand the role of these cells in different conditions and facilitate the development of safe and effective new treatments for diseases.

KEYWORDS:

Cell culture; FACS; Fibro/adipogenic progenitors; Mesenchymal stromal cell; Skeletal muscle

PMID:
28247350
DOI:
10.1007/978-1-4939-6771-1_9
[Indexed for MEDLINE]

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