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Appl Biochem Biotechnol. 2017 Aug;182(4):1540-1547. doi: 10.1007/s12010-017-2416-5. Epub 2017 Feb 28.

Differential Maturation of miR-17 ~ 92 Cluster Members in Human Cancer Cell Lines.

Author information

1
Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Science, Tabriz, Iran.
2
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3
Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran.
4
Department of Cell, Molecular, and Structural Biology, Miami University, Oxford, OH, USA.
5
Medical Laboratory Sciences Department, Faculty of Para-Medicine, Alborz University of Medical Sciences, Karaj, Iran.
6
Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
7
Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
8
Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Science, Tabriz, Iran. zarghamin@tbzmed.ac.ir.
9
Drug Applied Research Center, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. zarghamin@tbzmed.ac.ir.
10
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. hghanbarian@sbmu.ac.ir.
11
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. hghanbarian@sbmu.ac.ir.

Abstract

While some microRNAs are transcribed from a specific promoter, at least one third of human miRNA genes are clustered, wherein multiple miRNA genes are generated from a single primary transcript such as miR-17 ~ 92 cluster. Although six members of the cluster are generated from a single transcript, the mature level of each member may be diverse in various cell types. Here, we attempt to monitor the mature level of miR-17, miR-92a, and miR-20a from miR-17 ~ 92 cluster in blood (HL60 (human promyelocytic leukemia cells) and Jurkat) and breast (MDA-MB-231 and MCF-7) cancer cell lines. Interestingly, different mature levels of the miRNAs were observed in each cell line. While miR-20 was highly matured in HL60 and MDA-MB-231 cell lines, higher mature level of miR-92a was observed in Jurkat cell line compared to that of miR-20 and miR-17. Further, the mature level of miRNAs was also measured in normal and cancer cell lines. Although the mature level of miR-17 and miR-92a increased in HL60 and Jurkat cell lines, miR-20 expression showed an almost identical level in blood cancer cell lines compared to controls. Conversely, miR-20 mature level significantly increased in breast cancer cell lines whereas the expression level of miR-92a was comparable in MDA-MB-231, MCF-7, and MCF-10A cell lines.

KEYWORDS:

Cancer cell lines; Stem cell; miR-17 ~ 92 cluster; microRNA maturation

PMID:
28247308
DOI:
10.1007/s12010-017-2416-5
[Indexed for MEDLINE]

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